Detection of lens proteins in phaco tips after phacoemulsification

R M M A Nuijts; M Van Boekel; H Bloemendal; F Hiddema

doi:10.1016/s0886-3350(99)00241-2

Detection of lens proteins in phaco tips after phacoemulsification

R M M A Nuijts
, M Van Boekel
, H Bloemendal
, F Hiddema

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › Onderzoek › peer review

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PURPOSE: To determine the efficacy of cleaning procedures to remove water-soluble and water-insoluble lens proteins from phaco tips after phacoemulsification.

SETTING: Academic Hospital Maastricht, Department of Ophthalmology, University Eye Clinic, and Rotterdam Hospital, Rotterdam, The Netherlands.

METHODS: Detection of alpha A-crystallins, cytoskeletal lens proteins (vimentin), and lens-cell membranes (MP26) was performed on new and reused phaco tips with specific markers. To detect antibodies, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques were used.

RESULTS: Threshold detection levels for alpha A-crystallins, vimentin, and MP26 were 100, 43, and 108 ng, respectively. No detectable residues of water-soluble or water-insoluble lens proteins were found in outflow samples of routinely cleaned phaco tips or in outflow samples of phaco tips that were not cleaned after phacoemulsification. Urea effectively eliminated water-insoluble lens proteins from contaminated phaco tips.

CONCLUSION: After regular cleaning of phaco tips, no detectable lens protein residues were found in the outflow samples. Moreover, omitting the cleaning of phaco tips after phacoemulsification did not lead to detectable lens proteins in the outflow samples.

Originele taal-2	Engels
Pagina's (van-tot)	1510-4
Aantal pagina's	5
Tijdschrift	Journal of Cataract and Refractive Surgery
Volume	25
Nummer van het tijdschrift	11
DOI's	https://doi.org/10.1016/s0886-3350(99)00241-2
Status	Gepubliceerd - nov 1999

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10.1016/s0886-3350(99)00241-2

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@article{443ef2bee2e8448b822896a156559540,

title = "Detection of lens proteins in phaco tips after phacoemulsification",

abstract = "PURPOSE: To determine the efficacy of cleaning procedures to remove water-soluble and water-insoluble lens proteins from phaco tips after phacoemulsification.SETTING: Academic Hospital Maastricht, Department of Ophthalmology, University Eye Clinic, and Rotterdam Hospital, Rotterdam, The Netherlands.METHODS: Detection of alpha A-crystallins, cytoskeletal lens proteins (vimentin), and lens-cell membranes (MP26) was performed on new and reused phaco tips with specific markers. To detect antibodies, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques were used.RESULTS: Threshold detection levels for alpha A-crystallins, vimentin, and MP26 were 100, 43, and 108 ng, respectively. No detectable residues of water-soluble or water-insoluble lens proteins were found in outflow samples of routinely cleaned phaco tips or in outflow samples of phaco tips that were not cleaned after phacoemulsification. Urea effectively eliminated water-insoluble lens proteins from contaminated phaco tips.CONCLUSION: After regular cleaning of phaco tips, no detectable lens protein residues were found in the outflow samples. Moreover, omitting the cleaning of phaco tips after phacoemulsification did not lead to detectable lens proteins in the outflow samples.",

keywords = "Animals, Aquaporins, Biomarkers/analysis, Cattle, Disinfection/methods, Electrophoresis, Polyacrylamide Gel, Equipment Contamination/prevention \& control, Eye Proteins/analysis, Lens, Crystalline/chemistry, Membrane Glycoproteins, Phacoemulsification/instrumentation, Phosphoproteins/analysis, Vimentin/analysis",

author = "Nuijts, \{R M M A\} and \{Van Boekel\}, M and H Bloemendal and F Hiddema",

year = "1999",

month = nov,

doi = "10.1016/s0886-3350(99)00241-2",

language = "English",

volume = "25",

pages = "1510--4",

number = "11",

}

TY - JOUR

T1 - Detection of lens proteins in phaco tips after phacoemulsification

AU - Nuijts, R M M A

AU - Van Boekel, M

AU - Bloemendal, H

AU - Hiddema, F

PY - 1999/11

Y1 - 1999/11

N2 - PURPOSE: To determine the efficacy of cleaning procedures to remove water-soluble and water-insoluble lens proteins from phaco tips after phacoemulsification.SETTING: Academic Hospital Maastricht, Department of Ophthalmology, University Eye Clinic, and Rotterdam Hospital, Rotterdam, The Netherlands.METHODS: Detection of alpha A-crystallins, cytoskeletal lens proteins (vimentin), and lens-cell membranes (MP26) was performed on new and reused phaco tips with specific markers. To detect antibodies, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques were used.RESULTS: Threshold detection levels for alpha A-crystallins, vimentin, and MP26 were 100, 43, and 108 ng, respectively. No detectable residues of water-soluble or water-insoluble lens proteins were found in outflow samples of routinely cleaned phaco tips or in outflow samples of phaco tips that were not cleaned after phacoemulsification. Urea effectively eliminated water-insoluble lens proteins from contaminated phaco tips.CONCLUSION: After regular cleaning of phaco tips, no detectable lens protein residues were found in the outflow samples. Moreover, omitting the cleaning of phaco tips after phacoemulsification did not lead to detectable lens proteins in the outflow samples.

AB - PURPOSE: To determine the efficacy of cleaning procedures to remove water-soluble and water-insoluble lens proteins from phaco tips after phacoemulsification.SETTING: Academic Hospital Maastricht, Department of Ophthalmology, University Eye Clinic, and Rotterdam Hospital, Rotterdam, The Netherlands.METHODS: Detection of alpha A-crystallins, cytoskeletal lens proteins (vimentin), and lens-cell membranes (MP26) was performed on new and reused phaco tips with specific markers. To detect antibodies, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques were used.RESULTS: Threshold detection levels for alpha A-crystallins, vimentin, and MP26 were 100, 43, and 108 ng, respectively. No detectable residues of water-soluble or water-insoluble lens proteins were found in outflow samples of routinely cleaned phaco tips or in outflow samples of phaco tips that were not cleaned after phacoemulsification. Urea effectively eliminated water-insoluble lens proteins from contaminated phaco tips.CONCLUSION: After regular cleaning of phaco tips, no detectable lens protein residues were found in the outflow samples. Moreover, omitting the cleaning of phaco tips after phacoemulsification did not lead to detectable lens proteins in the outflow samples.

KW - Animals

KW - Aquaporins

KW - Biomarkers/analysis

KW - Cattle

KW - Disinfection/methods

KW - Electrophoresis, Polyacrylamide Gel

KW - Equipment Contamination/prevention & control

KW - Eye Proteins/analysis

KW - Lens, Crystalline/chemistry

KW - Membrane Glycoproteins

KW - Phacoemulsification/instrumentation

KW - Phosphoproteins/analysis

KW - Vimentin/analysis

U2 - 10.1016/s0886-3350(99)00241-2

DO - 10.1016/s0886-3350(99)00241-2

M3 - Article

C2 - 10569167

SN - 0886-3350

VL - 25

SP - 1510

EP - 1514

JO - Journal of Cataract and Refractive Surgery

JF - Journal of Cataract and Refractive Surgery

IS - 11

ER -

Detection of lens proteins in phaco tips after phacoemulsification

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