Analysis of local antibody production in the vitreous humor of patients with severe uveitis

G S Baarsma; L Luyendijk; A Kijlstra; J de Vries; E Peperkamp; D A Mertens; J C van Meurs

doi:10.1016/s0002-9394(14)76693-0

Analysis of local antibody production in the vitreous humor of patients with severe uveitis

G S Baarsma, L Luyendijk, A Kijlstra, J de Vries, E Peperkamp, D A Mertens, J C van Meurs

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › Onderzoek › peer review

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We analyzed the local antibody production in vitreous humor samples collected during vitrectomy in patients with severe vision-threatening uveitis. In 24 patients, paired serum and undiluted vitreous humor samples were collected and tested for antibodies against Toxoplasma gondii, herpes simplex virus, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, and Toxocara canis. Total IgG and the Goldmann-Witmer coefficient were determined. The initial diagnosis of ocular toxoplasmosis could be confirmed in six of the seven patients. The seventh patient showed a local antibody production against herpes simplex virus. One of the three patients with chronic panuveitis at initial diagnosis showed a local antibody production against T. gondii. These last two findings resulted in a change in medical treatment. Analysis of local antibody production in vitreous humor samples is a valuable diagnostic tool.

Originele taal-2	Engels
Pagina's (van-tot)	147-50
Aantal pagina's	4
Tijdschrift	American Journal of Ophthalmology
Volume	112
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/s0002-9394(14)76693-0
Status	Gepubliceerd - 15 aug. 1991

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10.1016/s0002-9394(14)76693-0

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@article{f91b7f929b72452a864f24a5741bee86,

title = "Analysis of local antibody production in the vitreous humor of patients with severe uveitis",

abstract = "We analyzed the local antibody production in vitreous humor samples collected during vitrectomy in patients with severe vision-threatening uveitis. In 24 patients, paired serum and undiluted vitreous humor samples were collected and tested for antibodies against Toxoplasma gondii, herpes simplex virus, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, and Toxocara canis. Total IgG and the Goldmann-Witmer coefficient were determined. The initial diagnosis of ocular toxoplasmosis could be confirmed in six of the seven patients. The seventh patient showed a local antibody production against herpes simplex virus. One of the three patients with chronic panuveitis at initial diagnosis showed a local antibody production against T. gondii. These last two findings resulted in a change in medical treatment. Analysis of local antibody production in vitreous humor samples is a valuable diagnostic tool.",

keywords = "Animals, Antibodies/analysis, Antibodies, Protozoan/analysis, Antibodies, Viral/analysis, Antibody Formation, Humans, Simplexvirus/immunology, Toxoplasma/immunology, Uveitis/immunology, Vitrectomy, Vitreous Body/immunology",

author = "Baarsma, \{G S\} and L Luyendijk and A Kijlstra and \{de Vries\}, J and E Peperkamp and Mertens, \{D A\} and \{van Meurs\}, \{J C\}",

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AU - Baarsma, G S

AU - Luyendijk, L

AU - Kijlstra, A

AU - de Vries, J

AU - Peperkamp, E

AU - Mertens, D A

AU - van Meurs, J C

PY - 1991/8/15

Y1 - 1991/8/15

N2 - We analyzed the local antibody production in vitreous humor samples collected during vitrectomy in patients with severe vision-threatening uveitis. In 24 patients, paired serum and undiluted vitreous humor samples were collected and tested for antibodies against Toxoplasma gondii, herpes simplex virus, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, and Toxocara canis. Total IgG and the Goldmann-Witmer coefficient were determined. The initial diagnosis of ocular toxoplasmosis could be confirmed in six of the seven patients. The seventh patient showed a local antibody production against herpes simplex virus. One of the three patients with chronic panuveitis at initial diagnosis showed a local antibody production against T. gondii. These last two findings resulted in a change in medical treatment. Analysis of local antibody production in vitreous humor samples is a valuable diagnostic tool.

AB - We analyzed the local antibody production in vitreous humor samples collected during vitrectomy in patients with severe vision-threatening uveitis. In 24 patients, paired serum and undiluted vitreous humor samples were collected and tested for antibodies against Toxoplasma gondii, herpes simplex virus, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, and Toxocara canis. Total IgG and the Goldmann-Witmer coefficient were determined. The initial diagnosis of ocular toxoplasmosis could be confirmed in six of the seven patients. The seventh patient showed a local antibody production against herpes simplex virus. One of the three patients with chronic panuveitis at initial diagnosis showed a local antibody production against T. gondii. These last two findings resulted in a change in medical treatment. Analysis of local antibody production in vitreous humor samples is a valuable diagnostic tool.

KW - Animals

KW - Antibodies/analysis

KW - Antibodies, Protozoan/analysis

KW - Antibodies, Viral/analysis

KW - Antibody Formation

KW - Humans

KW - Simplexvirus/immunology

KW - Toxoplasma/immunology

KW - Uveitis/immunology

KW - Vitrectomy

KW - Vitreous Body/immunology

U2 - 10.1016/s0002-9394(14)76693-0

DO - 10.1016/s0002-9394(14)76693-0

M3 - Article

C2 - 1651055

SN - 0002-9394

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SP - 147

EP - 150

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

IS - 2

ER -

Analysis of local antibody production in the vitreous humor of patients with severe uveitis

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