TY - JOUR
T1 - An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis
AU - Ugahary, Luana
AU - van de Sande, Wendy
AU - van Meurs, Jan C
AU - van Belkum, Alex
PY - 2004/11
Y1 - 2004/11
N2 - General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
AB - General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
KW - Anti-Infective Agents, Local/chemistry
KW - DNA, Bacterial/analysis
KW - Endophthalmitis/diagnosis
KW - Equipment Contamination
KW - Eye Infections, Bacterial/diagnosis
KW - Humans
KW - Povidone-Iodine/chemistry
KW - Pseudomonas/chemistry
KW - Vitrectomy
U2 - 10.1128/JCM.42.11.5403-5405.2004
DO - 10.1128/JCM.42.11.5403-5405.2004
M3 - Letter
C2 - 15528756
SN - 0095-1137
VL - 42
SP - 5403
EP - 5405
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 11
ER -