Vgamma9Vdelta2 T cells recovered from eyes of patients with Behçet's disease recognize non-peptide prenyl pyrophosphate antigens

Georges M G M Verjans, P Martin van Hagen, Alexander van der Kooi, Albert D M E Osterhaus, G Seerp Baarsma

Research output: Contribution to journalArticleResearchpeer-review


The phenotype and antigen-specificity of T cells expanded by mitogenic stimulation from intra-ocular fluid (IOF) samples of affected eyes of six Behçet's disease (BD) patients, and seven patients with other uveitis entities, were determined. High numbers of gammadelta T cells, predominantly Vgamma9Vdelta2 T cells, were only detected in the IOF-derived TCL of three BD patients. Whereas no TCL responded to heat shock protein (HSP) 65 kDa, reactivity to isopentyl pyrophosphate (IPP) and related non-peptide prenyl pyrophosphates (PPP) was restricted to the gammadelta T cell containing TCL. Upon IPP stimulation, these TCL secreted IFN-gamma but no IL-4. By single-cell analysis of intracellular IFN-gamma production and CD69 expression the IOF-derived IPP-specific T cells were identified as CD4(-)CD8(-) gammadelta T cells. The data presented suggest the infiltration of PPP-specific Vgamma9Vdelta2 Th1-like cells into the eye of BD patients with uveitis.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalJournal of Neuroimmunology
Issue number1-2
Publication statusPublished - Sept 2002


  • Adult
  • Aged
  • Antigen Presentation/immunology
  • Antigens, CD/immunology
  • Antigens, Differentiation, T-Lymphocyte/immunology
  • Aqueous Humor/immunology
  • Behcet Syndrome/immunology
  • CD3 Complex/immunology
  • CD4-CD8 Ratio
  • Chemotaxis, Leukocyte/immunology
  • Child
  • Cytokines/immunology
  • Diphosphates/immunology
  • Epitopes/immunology
  • Eye/immunology
  • Female
  • Flow Cytometry
  • Humans
  • Interferon-gamma/immunology
  • Lectins, C-Type
  • Male
  • Middle Aged
  • Protein Prenylation/immunology
  • Receptors, Antigen, T-Cell, gamma-delta/immunology
  • T-Lymphocytes/immunology


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