Trypan blue staining in vitreoretinal surgery

Francisco Aguilera Teba; Andreas Mohr; Claus Eckardt; David Wong; Shunji Kusaka; Brian C Joondeph; Eric J Feron; Peter Stalmans; Koen Van Overdam; Gerrit R J Melles

doi:10.1016/s0161-6420(03)00716-4

Trypan blue staining in vitreoretinal surgery

Francisco Aguilera Teba, Andreas Mohr, Claus Eckardt, David Wong, Shunji Kusaka, Brian C Joondeph, Eric J Feron, Peter Stalmans, Koen Van Overdam, Gerrit R J Melles

Research output: Contribution to journal › Article › Professional › peer-review

Abstract

PURPOSE: To evaluate the efficacy of trypan blue for staining the internal limiting membrane (ILM) and epiretinal membranes (ERM) in vitreoretinal surgery.

DESIGN: Prospective noncomparative case series.

PARTICIPANTS: Fifty eyes of 50 patients with macular pucker (n = 22), macular hole (n = 18), or a combination (n = 2), proliferative vitreoretinopathy (n = 5), or diabetic retinopathy (n = 3).

METHODS: Trypan blue 0.2% was used to stain the ILM or ERM during vitreoretinal surgery.

MAIN OUTCOME MEASURES: The intraoperative visibility of the membranes was scored as poor, moderate, good, or excellent.

RESULTS: The application of trypan blue onto the ILM or the ERM resulted in a useful bluish staining, facilitating the identification, delineation, and removal of the membranes in all surgeries. No residual staining or adverse effects related to the dye were observed.

CONCLUSIONS: Trypan blue stains both ILM and ERM and might be an useful tool in vitreoretinal surgery.

Original language	English
Pages (from-to)	2409-12
Number of pages	4
Journal	Ophthalmology
Volume	110
Issue number	12
DOIs	https://doi.org/10.1016/s0161-6420(03)00716-4
Publication status	Published - Dec 2003

Keywords

Adult
Aged
Aged, 80 and over
Basement Membrane/pathology
Coloring Agents
Epiretinal Membrane/diagnosis
Eye Diseases/surgery
Female
Humans
Male
Middle Aged
Prospective Studies
Retinal Diseases/surgery
Staining and Labeling/methods
Trypan Blue
Vitreous Body/surgery

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10.1016/s0161-6420(03)00716-4

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title = "Trypan blue staining in vitreoretinal surgery",

abstract = "PURPOSE: To evaluate the efficacy of trypan blue for staining the internal limiting membrane (ILM) and epiretinal membranes (ERM) in vitreoretinal surgery.DESIGN: Prospective noncomparative case series.PARTICIPANTS: Fifty eyes of 50 patients with macular pucker (n = 22), macular hole (n = 18), or a combination (n = 2), proliferative vitreoretinopathy (n = 5), or diabetic retinopathy (n = 3).METHODS: Trypan blue 0.2% was used to stain the ILM or ERM during vitreoretinal surgery.MAIN OUTCOME MEASURES: The intraoperative visibility of the membranes was scored as poor, moderate, good, or excellent.RESULTS: The application of trypan blue onto the ILM or the ERM resulted in a useful bluish staining, facilitating the identification, delineation, and removal of the membranes in all surgeries. No residual staining or adverse effects related to the dye were observed.CONCLUSIONS: Trypan blue stains both ILM and ERM and might be an useful tool in vitreoretinal surgery.",

keywords = "Adult, Aged, Aged, 80 and over, Basement Membrane/pathology, Coloring Agents, Epiretinal Membrane/diagnosis, Eye Diseases/surgery, Female, Humans, Male, Middle Aged, Prospective Studies, Retinal Diseases/surgery, Staining and Labeling/methods, Trypan Blue, Vitreous Body/surgery",

author = "Teba, {Francisco Aguilera} and Andreas Mohr and Claus Eckardt and David Wong and Shunji Kusaka and Joondeph, {Brian C} and Feron, {Eric J} and Peter Stalmans and {Van Overdam}, Koen and Melles, {Gerrit R J}",

year = "2003",

month = dec,

doi = "10.1016/s0161-6420(03)00716-4",

language = "English",

volume = "110",

pages = "2409--12",

number = "12",

}

TY - JOUR

T1 - Trypan blue staining in vitreoretinal surgery

AU - Teba, Francisco Aguilera

AU - Mohr, Andreas

AU - Eckardt, Claus

AU - Wong, David

AU - Kusaka, Shunji

AU - Joondeph, Brian C

AU - Feron, Eric J

AU - Stalmans, Peter

AU - Van Overdam, Koen

AU - Melles, Gerrit R J

PY - 2003/12

Y1 - 2003/12

N2 - PURPOSE: To evaluate the efficacy of trypan blue for staining the internal limiting membrane (ILM) and epiretinal membranes (ERM) in vitreoretinal surgery.DESIGN: Prospective noncomparative case series.PARTICIPANTS: Fifty eyes of 50 patients with macular pucker (n = 22), macular hole (n = 18), or a combination (n = 2), proliferative vitreoretinopathy (n = 5), or diabetic retinopathy (n = 3).METHODS: Trypan blue 0.2% was used to stain the ILM or ERM during vitreoretinal surgery.MAIN OUTCOME MEASURES: The intraoperative visibility of the membranes was scored as poor, moderate, good, or excellent.RESULTS: The application of trypan blue onto the ILM or the ERM resulted in a useful bluish staining, facilitating the identification, delineation, and removal of the membranes in all surgeries. No residual staining or adverse effects related to the dye were observed.CONCLUSIONS: Trypan blue stains both ILM and ERM and might be an useful tool in vitreoretinal surgery.

AB - PURPOSE: To evaluate the efficacy of trypan blue for staining the internal limiting membrane (ILM) and epiretinal membranes (ERM) in vitreoretinal surgery.DESIGN: Prospective noncomparative case series.PARTICIPANTS: Fifty eyes of 50 patients with macular pucker (n = 22), macular hole (n = 18), or a combination (n = 2), proliferative vitreoretinopathy (n = 5), or diabetic retinopathy (n = 3).METHODS: Trypan blue 0.2% was used to stain the ILM or ERM during vitreoretinal surgery.MAIN OUTCOME MEASURES: The intraoperative visibility of the membranes was scored as poor, moderate, good, or excellent.RESULTS: The application of trypan blue onto the ILM or the ERM resulted in a useful bluish staining, facilitating the identification, delineation, and removal of the membranes in all surgeries. No residual staining or adverse effects related to the dye were observed.CONCLUSIONS: Trypan blue stains both ILM and ERM and might be an useful tool in vitreoretinal surgery.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Basement Membrane/pathology

KW - Coloring Agents

KW - Epiretinal Membrane/diagnosis

KW - Eye Diseases/surgery

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Prospective Studies

KW - Retinal Diseases/surgery

KW - Staining and Labeling/methods

KW - Trypan Blue

KW - Vitreous Body/surgery

U2 - 10.1016/s0161-6420(03)00716-4

DO - 10.1016/s0161-6420(03)00716-4

M3 - Article

C2 - 14644726

SN - 0161-6420

VL - 110

SP - 2409

EP - 2412

JO - Ophthalmology

JF - Ophthalmology

IS - 12

ER -

Trypan blue staining in vitreoretinal surgery

Abstract

Keywords

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