Trypan blue staining in vitreoretinal surgery

Francisco Aguilera Teba, Andreas Mohr, Claus Eckardt, David Wong, Shunji Kusaka, Brian C Joondeph, Eric J Feron, Peter Stalmans, Koen Van Overdam, Gerrit R J Melles

    Research output: Contribution to journalArticleProfessionalpeer-review


    PURPOSE: To evaluate the efficacy of trypan blue for staining the internal limiting membrane (ILM) and epiretinal membranes (ERM) in vitreoretinal surgery.

    DESIGN: Prospective noncomparative case series.

    PARTICIPANTS: Fifty eyes of 50 patients with macular pucker (n = 22), macular hole (n = 18), or a combination (n = 2), proliferative vitreoretinopathy (n = 5), or diabetic retinopathy (n = 3).

    METHODS: Trypan blue 0.2% was used to stain the ILM or ERM during vitreoretinal surgery.

    MAIN OUTCOME MEASURES: The intraoperative visibility of the membranes was scored as poor, moderate, good, or excellent.

    RESULTS: The application of trypan blue onto the ILM or the ERM resulted in a useful bluish staining, facilitating the identification, delineation, and removal of the membranes in all surgeries. No residual staining or adverse effects related to the dye were observed.

    CONCLUSIONS: Trypan blue stains both ILM and ERM and might be an useful tool in vitreoretinal surgery.

    Original languageEnglish
    Pages (from-to)2409-12
    Number of pages4
    Issue number12
    Publication statusPublished - Dec 2003


    • Adult
    • Aged
    • Aged, 80 and over
    • Basement Membrane/pathology
    • Coloring Agents
    • Epiretinal Membrane/diagnosis
    • Eye Diseases/surgery
    • Female
    • Humans
    • Male
    • Middle Aged
    • Prospective Studies
    • Retinal Diseases/surgery
    • Staining and Labeling/methods
    • Trypan Blue
    • Vitreous Body/surgery


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