Trypan blue not toxic for retinal pigment epithelium in vitro

Peter Stalmans; Elisabeth H Van Aken; Gerrit Melles; Marc Veckeneer; Eric J Feron; Ingeborg Stalmans

doi:10.1016/s0002-9394(02)01891-3

Trypan blue not toxic for retinal pigment epithelium in vitro

Peter Stalmans
, Elisabeth H Van Aken
, Gerrit Melles
, Marc Veckeneer
, Eric J Feron
, Ingeborg Stalmans

Vitreoretinal surgery

Research output: Contribution to journal › Article › Research › peer-review

Abstract

PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.

DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.

METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.

RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.

CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.

Original language	English
Pages (from-to)	234-236
Number of pages	3
Journal	American Journal of Ophthalmology
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/s0002-9394(02)01891-3
Publication status	Published - Feb 2003

Keywords

Cell Survival/drug effects
Cells, Cultured
Coloring Agents/pharmacology
Humans
Microscopy, Confocal
Pigment Epithelium of Eye/cytology
Trypan Blue/pharmacology

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10.1016/s0002-9394(02)01891-3

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title = "Trypan blue not toxic for retinal pigment epithelium in vitro",

abstract = "PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06\%, 0.15\%, 0.30\%), and cell viability was confocally measured.RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.",

keywords = "Cell Survival/drug effects, Cells, Cultured, Coloring Agents/pharmacology, Humans, Microscopy, Confocal, Pigment Epithelium of Eye/cytology, Trypan Blue/pharmacology",

author = "Peter Stalmans and \{Van Aken\}, \{Elisabeth H\} and Gerrit Melles and Marc Veckeneer and Feron, \{Eric J\} and Ingeborg Stalmans",

year = "2003",

month = feb,

doi = "10.1016/s0002-9394(02)01891-3",

language = "English",

volume = "135",

pages = "234--236",

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TY - JOUR

T1 - Trypan blue not toxic for retinal pigment epithelium in vitro

AU - Stalmans, Peter

AU - Van Aken, Elisabeth H

AU - Melles, Gerrit

AU - Veckeneer, Marc

AU - Feron, Eric J

AU - Stalmans, Ingeborg

PY - 2003/2

Y1 - 2003/2

N2 - PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.

AB - PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.

KW - Cell Survival/drug effects

KW - Cells, Cultured

KW - Coloring Agents/pharmacology

KW - Humans

KW - Microscopy, Confocal

KW - Pigment Epithelium of Eye/cytology

KW - Trypan Blue/pharmacology

U2 - 10.1016/s0002-9394(02)01891-3

DO - 10.1016/s0002-9394(02)01891-3

M3 - Article

C2 - 12566035

SN - 0002-9394

VL - 135

SP - 234

EP - 236

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

IS - 2

ER -

Trypan blue not toxic for retinal pigment epithelium in vitro

Abstract

Keywords

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