PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.
DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.
METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.
RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.
CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.
|Number of pages||3|
|Journal||American Journal of Ophthalmology|
|Publication status||Published - Feb 2003|
- Cell Survival/drug effects
- Cells, Cultured
- Coloring Agents/pharmacology
- Microscopy, Confocal
- Pigment Epithelium of Eye/cytology
- Trypan Blue/pharmacology