Trypan blue not toxic for retinal pigment epithelium in vitro

Peter Stalmans, Elisabeth H Van Aken, Gerrit Melles, Marc Veckeneer, Eric J Feron, Ingeborg Stalmans

Research output: Contribution to journalArticleResearchpeer-review


PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.

DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.

METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.

RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.

CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.

Original languageEnglish
Pages (from-to)234-236
Number of pages3
JournalAmerican Journal of Ophthalmology
Issue number2
Publication statusPublished - Feb 2003


  • Cell Survival/drug effects
  • Cells, Cultured
  • Coloring Agents/pharmacology
  • Humans
  • Microscopy, Confocal
  • Pigment Epithelium of Eye/cytology
  • Trypan Blue/pharmacology


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