Thrombin Generation in Vitreous and Subretinal Fluid of Patients with Retinal Detachment

Verena C Mulder; Jeroen Bastiaans; Cornelis J M van Leuven; Jan C van Meurs; Cornelis Kluft

doi:10.1159/000487757

Thrombin Generation in Vitreous and Subretinal Fluid of Patients with Retinal Detachment

Verena C Mulder, Jeroen Bastiaans, Cornelis J M van Leuven, Jan C van Meurs, Cornelis Kluft

Research output: Contribution to journal › Article › Research › peer-review

Abstract

PURPOSE: To measure prothrombin fragments (F1+2) and thrombin-antithrombin complex (TAT) in vitreous and subretinal fluid (SRF) of rhegmatogenous retinal detachment (RRD) patients and to validate and further specify our earlier finding of increased thrombin activity in patients with proliferative vitreoretinopathy (PVR).

METHODS: F1+2 and TAT were measured in 31 vitreous and 16 SRF samples using the Enzygnost® immunoassays.

RESULTS: We found significant levels of F1+2 and TAT in the vitreous of all patients with RRD compared to patients with macular hole or macular pucker. However, there was no significant difference between patients who would develop PVR in the future, had established PVR, and patients with uncomplicated RRD both in vitreous concentrations of F1+2 (Kruskal-Wallis p = 0.963) and TAT (p = 0.516).

CONCLUSION: The analysis of F1+2 and TAT confirmed significant thrombin generation in both vitreous and SRF of patients with RRD. An imbalance between the thrombin regulation mechanisms TAT and α2-macroglobulin possibly explains the difference from our previous findings.

Original language	English
Pages (from-to)	23-28
Number of pages	6
Journal	Ophthalmologica
Volume	240
Issue number	1
DOIs	https://doi.org/10.1159/000487757
Publication status	Published - 2018

Keywords

Aged
Antithrombin III/metabolism
Female
Humans
Immunoenzyme Techniques
Male
Middle Aged
Peptide Fragments/metabolism
Peptide Hydrolases/metabolism
Prothrombin/metabolism
Retinal Detachment/metabolism
Retinal Perforations/metabolism
Subretinal Fluid/metabolism
Vitrectomy
Vitreoretinopathy, Proliferative/metabolism
Vitreous Body/metabolism

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10.1159/000487757

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@article{f718c0f5b4474bbba79a52814913548a,

title = "Thrombin Generation in Vitreous and Subretinal Fluid of Patients with Retinal Detachment",

abstract = "PURPOSE: To measure prothrombin fragments (F1+2) and thrombin-antithrombin complex (TAT) in vitreous and subretinal fluid (SRF) of rhegmatogenous retinal detachment (RRD) patients and to validate and further specify our earlier finding of increased thrombin activity in patients with proliferative vitreoretinopathy (PVR).METHODS: F1+2 and TAT were measured in 31 vitreous and 16 SRF samples using the Enzygnost{\textregistered} immunoassays.RESULTS: We found significant levels of F1+2 and TAT in the vitreous of all patients with RRD compared to patients with macular hole or macular pucker. However, there was no significant difference between patients who would develop PVR in the future, had established PVR, and patients with uncomplicated RRD both in vitreous concentrations of F1+2 (Kruskal-Wallis p = 0.963) and TAT (p = 0.516).CONCLUSION: The analysis of F1+2 and TAT confirmed significant thrombin generation in both vitreous and SRF of patients with RRD. An imbalance between the thrombin regulation mechanisms TAT and α2-macroglobulin possibly explains the difference from our previous findings.",

keywords = "Aged, Antithrombin III/metabolism, Female, Humans, Immunoenzyme Techniques, Male, Middle Aged, Peptide Fragments/metabolism, Peptide Hydrolases/metabolism, Prothrombin/metabolism, Retinal Detachment/metabolism, Retinal Perforations/metabolism, Subretinal Fluid/metabolism, Vitrectomy, Vitreoretinopathy, Proliferative/metabolism, Vitreous Body/metabolism",

author = "Mulder, {Verena C} and Jeroen Bastiaans and {van Leuven}, {Cornelis J M} and {van Meurs}, {Jan C} and Cornelis Kluft",

note = "{\textcopyright} 2018 The Author(s) Published by S. Karger AG, Basel.",

year = "2018",

doi = "10.1159/000487757",

language = "English",

volume = "240",

pages = "23--28",

number = "1",

}

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T1 - Thrombin Generation in Vitreous and Subretinal Fluid of Patients with Retinal Detachment

AU - Mulder, Verena C

AU - Bastiaans, Jeroen

AU - van Leuven, Cornelis J M

AU - van Meurs, Jan C

AU - Kluft, Cornelis

PY - 2018

Y1 - 2018

N2 - PURPOSE: To measure prothrombin fragments (F1+2) and thrombin-antithrombin complex (TAT) in vitreous and subretinal fluid (SRF) of rhegmatogenous retinal detachment (RRD) patients and to validate and further specify our earlier finding of increased thrombin activity in patients with proliferative vitreoretinopathy (PVR).METHODS: F1+2 and TAT were measured in 31 vitreous and 16 SRF samples using the Enzygnost® immunoassays.RESULTS: We found significant levels of F1+2 and TAT in the vitreous of all patients with RRD compared to patients with macular hole or macular pucker. However, there was no significant difference between patients who would develop PVR in the future, had established PVR, and patients with uncomplicated RRD both in vitreous concentrations of F1+2 (Kruskal-Wallis p = 0.963) and TAT (p = 0.516).CONCLUSION: The analysis of F1+2 and TAT confirmed significant thrombin generation in both vitreous and SRF of patients with RRD. An imbalance between the thrombin regulation mechanisms TAT and α2-macroglobulin possibly explains the difference from our previous findings.

AB - PURPOSE: To measure prothrombin fragments (F1+2) and thrombin-antithrombin complex (TAT) in vitreous and subretinal fluid (SRF) of rhegmatogenous retinal detachment (RRD) patients and to validate and further specify our earlier finding of increased thrombin activity in patients with proliferative vitreoretinopathy (PVR).METHODS: F1+2 and TAT were measured in 31 vitreous and 16 SRF samples using the Enzygnost® immunoassays.RESULTS: We found significant levels of F1+2 and TAT in the vitreous of all patients with RRD compared to patients with macular hole or macular pucker. However, there was no significant difference between patients who would develop PVR in the future, had established PVR, and patients with uncomplicated RRD both in vitreous concentrations of F1+2 (Kruskal-Wallis p = 0.963) and TAT (p = 0.516).CONCLUSION: The analysis of F1+2 and TAT confirmed significant thrombin generation in both vitreous and SRF of patients with RRD. An imbalance between the thrombin regulation mechanisms TAT and α2-macroglobulin possibly explains the difference from our previous findings.

KW - Aged

KW - Antithrombin III/metabolism

KW - Female

KW - Humans

KW - Immunoenzyme Techniques

KW - Male

KW - Middle Aged

KW - Peptide Fragments/metabolism

KW - Peptide Hydrolases/metabolism

KW - Prothrombin/metabolism

KW - Retinal Detachment/metabolism

KW - Retinal Perforations/metabolism

KW - Subretinal Fluid/metabolism

KW - Vitrectomy

KW - Vitreoretinopathy, Proliferative/metabolism

KW - Vitreous Body/metabolism

U2 - 10.1159/000487757

DO - 10.1159/000487757

M3 - Article

C2 - 29617690

SN - 0030-3755

VL - 240

SP - 23

EP - 28

JO - Ophthalmologica

JF - Ophthalmologica

IS - 1

ER -

Thrombin Generation in Vitreous and Subretinal Fluid of Patients with Retinal Detachment

Abstract

Keywords

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