Thrombin Generation in Vitreous and Subretinal Fluid of Patients with Retinal Detachment

Verena C Mulder, Jeroen Bastiaans, Cornelis J M van Leuven, Jan C van Meurs, Cornelis Kluft

Research output: Contribution to journalArticleResearchpeer-review


PURPOSE: To measure prothrombin fragments (F1+2) and thrombin-antithrombin complex (TAT) in vitreous and subretinal fluid (SRF) of rhegmatogenous retinal detachment (RRD) patients and to validate and further specify our earlier finding of increased thrombin activity in patients with proliferative vitreoretinopathy (PVR).

METHODS: F1+2 and TAT were measured in 31 vitreous and 16 SRF samples using the Enzygnost® immunoassays.

RESULTS: We found significant levels of F1+2 and TAT in the vitreous of all patients with RRD compared to patients with macular hole or macular pucker. However, there was no significant difference between patients who would develop PVR in the future, had established PVR, and patients with uncomplicated RRD both in vitreous concentrations of F1+2 (Kruskal-Wallis p = 0.963) and TAT (p = 0.516).

CONCLUSION: The analysis of F1+2 and TAT confirmed significant thrombin generation in both vitreous and SRF of patients with RRD. An imbalance between the thrombin regulation mechanisms TAT and α2-macroglobulin possibly explains the difference from our previous findings.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
Issue number1
Publication statusPublished - 2018


  • Aged
  • Antithrombin III/metabolism
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Middle Aged
  • Peptide Fragments/metabolism
  • Peptide Hydrolases/metabolism
  • Prothrombin/metabolism
  • Retinal Detachment/metabolism
  • Retinal Perforations/metabolism
  • Subretinal Fluid/metabolism
  • Vitrectomy
  • Vitreoretinopathy, Proliferative/metabolism
  • Vitreous Body/metabolism


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