Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis

R Duan, J M van Dun, L Remeijer, M Siemerink, P G H Mulder, P Norberg, A D M E Osterhaus, G M G M Verjans

Research output: Contribution to journalArticleResearchpeer-review


AIM: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).

METHODS: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.

RESULTS: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.

CONCLUSIONS: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.

Original languageEnglish
Pages (from-to)1195-200
Number of pages6
JournalBritish Journal of Ophthalmology
Issue number9
Publication statusPublished - Sept 2008


  • Aged
  • Cohort Studies
  • DNA, Viral/genetics
  • Female
  • Genotype
  • Herpesvirus 1, Human/classification
  • Humans
  • Keratitis, Herpetic/virology
  • Male
  • Middle Aged
  • Netherlands
  • Polymorphism, Restriction Fragment Length
  • Viral Envelope Proteins/analysis


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