Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis

R Duan; J M van Dun; L Remeijer; M Siemerink; P G H Mulder; P Norberg; A D M E Osterhaus; G M G M Verjans

doi:10.1136/bjo.2007.136044

Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis

R Duan, J M van Dun, L Remeijer, M Siemerink, P G H Mulder, P Norberg, A D M E Osterhaus, G M G M Verjans

Cornea

Research output: Contribution to journal › Article › Research › peer-review

Abstract

AIM: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).

METHODS: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.

RESULTS: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.

CONCLUSIONS: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.

Original language	English
Pages (from-to)	1195-200
Number of pages	6
Journal	British Journal of Ophthalmology
Volume	92
Issue number	9
DOIs	https://doi.org/10.1136/bjo.2007.136044
Publication status	Published - Sept 2008

Keywords

Aged
Cohort Studies
DNA, Viral/genetics
Female
Genotype
Herpesvirus 1, Human/classification
Humans
Keratitis, Herpetic/virology
Male
Middle Aged
Netherlands
Polymorphism, Restriction Fragment Length
Viral Envelope Proteins/analysis

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10.1136/bjo.2007.136044

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title = "Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis",

abstract = "AIM: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).METHODS: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.RESULTS: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.CONCLUSIONS: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.",

keywords = "Aged, Cohort Studies, DNA, Viral/genetics, Female, Genotype, Herpesvirus 1, Human/classification, Humans, Keratitis, Herpetic/virology, Male, Middle Aged, Netherlands, Polymorphism, Restriction Fragment Length, Viral Envelope Proteins/analysis",

author = "R Duan and {van Dun}, {J M} and L Remeijer and M Siemerink and Mulder, {P G H} and P Norberg and Osterhaus, {A D M E} and Verjans, {G M G M}",

year = "2008",

month = sep,

doi = "10.1136/bjo.2007.136044",

language = "English",

volume = "92",

pages = "1195--200",

number = "9",

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TY - JOUR

T1 - Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis

AU - Duan, R

AU - van Dun, J M

AU - Remeijer, L

AU - Siemerink, M

AU - Mulder, P G H

AU - Norberg, P

AU - Osterhaus, A D M E

AU - Verjans, G M G M

PY - 2008/9

Y1 - 2008/9

N2 - AIM: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).METHODS: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.RESULTS: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.CONCLUSIONS: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.

AB - AIM: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).METHODS: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.RESULTS: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.CONCLUSIONS: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.

KW - Aged

KW - Cohort Studies

KW - DNA, Viral/genetics

KW - Female

KW - Genotype

KW - Herpesvirus 1, Human/classification

KW - Humans

KW - Keratitis, Herpetic/virology

KW - Male

KW - Middle Aged

KW - Netherlands

KW - Polymorphism, Restriction Fragment Length

KW - Viral Envelope Proteins/analysis

U2 - 10.1136/bjo.2007.136044

DO - 10.1136/bjo.2007.136044

M3 - Article

C2 - 18617539

SN - 0007-1161

VL - 92

SP - 1195

EP - 1200

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

IS - 9

ER -

Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis

Abstract

Keywords

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