Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes

M Veckeneer, K van Overdam, J Monzer, K Kobuch, W van Marle, H Spekreijse, J van Meurs

Research output: Contribution to journalArticleResearchpeer-review

Abstract

BACKGROUND: To evaluate the ocular toxicity of trypan blue (TB) injected into the vitreous cavity of rabbit eyes. TB is a dye that could be useful for staining epiretinal membranes during vitrectomy surgery.

METHODS: Ten New Zealand White (NZW) rabbits underwent gas-compression vitrectomy. Rabbits were divided into three groups to receive injections of 0.1 ml basic salt solution, 0.1 ml of a 0.06% TB solution or 0.1 ml of a 0.2% TB solution. Ocular toxicity was assessed by slit-lamp biomicroscopy, ophthalmoscopy, electroretinography and histology.

RESULTS: Transient posterior capsule opacification was noted in all animals. No significant reductions in a-wave or b-wave amplitudes were found in any of the animals. Light and electron microscopic examination of the inferior retina in the 0.2% TB-treated eyes showed damaged photoreceptors and marked disorganization. Immunohistochemical staining for rhodopsin was strongly reduced in those sections and staining for proliferation with Ki-67 was positive. No histological abnormalities were found in the upper retina of the 0.2% TB-treated eyes or in any part of the retina of the 0.06% TB-treated or control eyes. No histological abnormalities were found in any of the anterior chamber angle specimens.

CONCLUSIONS: Although no signs of toxicity were found after the prolonged presence of TB at a concentration of 0.06% in the vitreous cavity of rabbit eyes, marked damage occurred in the lower retina of 0.2% TB-treated eyes. The short-term presence of TB at a concentration of 0.06% in the vitreous cavity is harmless to the rabbit eye but a higher concentration of TB could be unsafe.

Original languageEnglish
Pages (from-to)698-704
Number of pages7
JournalGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
Volume239
Issue number9
DOIs
Publication statusPublished - Sep 2001

Keywords

  • Animals
  • Cataract/chemically induced
  • Coloring Agents/toxicity
  • Electroretinography
  • Immunohistochemistry
  • Ki-67 Antigen/metabolism
  • Lens Capsule, Crystalline/drug effects
  • Ophthalmic Solutions
  • Rabbits
  • Retina/drug effects
  • Retinal Degeneration/chemically induced
  • Rhodopsin/metabolism
  • Trypan Blue/toxicity
  • Vitrectomy
  • Vitreous Body/drug effects

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