Novel 'heavy' dyes for retinal membrane staining during macular surgery: multicenter clinical assessment

Marc Veckeneer; Andreas Mohr; Essam Alharthi; Rajvardhan Azad; Ziad F Bashshur; Enrico Bertelli; Riad A Bejjani; Brahim Bouassida; Dan Bourla; Iñigo Corcóstegui Crespo; Charbel Fahed; Faisal Fayyad; Marco Mura; Jerzy Nawrocki; Kelvin Rivett; Gabor B Scharioth; Dmitry O Shkvorchenko; Peter Szurman; Hein Van Wijck; Ian Y Wong; David S H Wong; Johannes Frank; Silke Oellerich; Marieke Bruinsma; Gerrit R J Melles

doi:10.1111/aos.12208

Novel 'heavy' dyes for retinal membrane staining during macular surgery: multicenter clinical assessment

Marc Veckeneer, Andreas Mohr, Essam Alharthi, Rajvardhan Azad, Ziad F Bashshur, Enrico Bertelli, Riad A Bejjani, Brahim Bouassida, Dan Bourla, Iñigo Corcóstegui Crespo, Charbel Fahed, Faisal Fayyad, Marco Mura, Jerzy Nawrocki, Kelvin Rivett, Gabor B Scharioth, Dmitry O Shkvorchenko, Peter Szurman, Hein Van Wijck, Ian Y WongDavid S H Wong, Johannes Frank, Silke Oellerich, Marieke Bruinsma, Gerrit R J Melles

Vitreoretinal surgery

Research output: Contribution to journal › Article › Research › peer-review

Abstract

PURPOSE: To evaluate the feasibility of two novel 'heavy' dye solutions for staining the internal limiting membrane (ILM) and epiretinal membranes (ERMs), without the need for a prior fluid-air exchange, during macular surgery.

METHODS: In this prospective nonrandomized multicenter cohort study, the high molecular weight dyes ILM-Blue™ [0.025% brilliant blue G, 4% polyethylene glycol (PEG)] and MembraneBlue-Dual™ (0.15% trypan blue, 0.025% brilliant blue G, 4% PEG) were randomly used in vitrectomy surgeries for macular disease in 127 eyes of 127 patients. Dye enhanced membrane visualization of the ILM and ERMs, 'ease of membrane peeling', visually detectable perioperative retinal damage, postoperative best-corrected visual acuity (BCVA), dye remnants and other unexpected clinical events were documented by 21 surgeons.

RESULTS: All surgeries were uneventful, and a clear bluish staining, facilitating the identification, delineation and removal of the ILM and ERMs, was reported in all but five cases. None of the surgeries required a fluid-air exchange to assist the dye application. BCVA at 1 month after surgery improved in 83% of the eyes in the MembraneBlue-Dual™ group and in 88% in the ILM-Blue™ group. No dye remnants were detected by ophthalmoscopy, and no retinal adverse effects related to the surgery or use of the dyes were observed.

CONCLUSION: The 'heavy' dye solutions ILM-Blue™ and MembraneBlue-Dual™ can be injected into a fluid-filled vitreous cavity and may facilitate staining and removal of the ILM and/or ERMs in macular surgery without an additional fluid-air exchange.

Original language	English
Pages (from-to)	339-44
Number of pages	6
Journal	Acta Ophthalmologica
Volume	92
Issue number	4
DOIs	https://doi.org/10.1111/aos.12208
Publication status	Published - Jun 2014

Keywords

Aged
Basement Membrane/pathology
Coloring Agents
Drug Combinations
Epiretinal Membrane/diagnosis
Female
Humans
Indicators and Reagents
Intraocular Pressure/drug effects
Male
Polyethylene Glycols
Prospective Studies
Retinal Diseases/surgery
Rosaniline Dyes
Staining and Labeling/methods
Trypan Blue
Visual Acuity/drug effects
Vitrectomy

Access to Document

10.1111/aos.12208

Cite this

Veckeneer, M., Mohr, A., Alharthi, E., Azad, R., Bashshur, Z. F., Bertelli, E., Bejjani, R. A., Bouassida, B., Bourla, D., Crespo, I. C., Fahed, C., Fayyad, F., Mura, M., Nawrocki, J., Rivett, K., Scharioth, G. B., Shkvorchenko, D. O., Szurman, P., Van Wijck, H., ... Melles, G. R. J. (2014). Novel 'heavy' dyes for retinal membrane staining during macular surgery: multicenter clinical assessment. Acta Ophthalmologica, 92(4), 339-44. https://doi.org/10.1111/aos.12208

@article{50caa297130541b5a2b1d85ed2b447fc,

title = "Novel 'heavy' dyes for retinal membrane staining during macular surgery: multicenter clinical assessment",

abstract = "PURPOSE: To evaluate the feasibility of two novel 'heavy' dye solutions for staining the internal limiting membrane (ILM) and epiretinal membranes (ERMs), without the need for a prior fluid-air exchange, during macular surgery.METHODS: In this prospective nonrandomized multicenter cohort study, the high molecular weight dyes ILM-Blue{\texttrademark} [0.025% brilliant blue G, 4% polyethylene glycol (PEG)] and MembraneBlue-Dual{\texttrademark} (0.15% trypan blue, 0.025% brilliant blue G, 4% PEG) were randomly used in vitrectomy surgeries for macular disease in 127 eyes of 127 patients. Dye enhanced membrane visualization of the ILM and ERMs, 'ease of membrane peeling', visually detectable perioperative retinal damage, postoperative best-corrected visual acuity (BCVA), dye remnants and other unexpected clinical events were documented by 21 surgeons.RESULTS: All surgeries were uneventful, and a clear bluish staining, facilitating the identification, delineation and removal of the ILM and ERMs, was reported in all but five cases. None of the surgeries required a fluid-air exchange to assist the dye application. BCVA at 1 month after surgery improved in 83% of the eyes in the MembraneBlue-Dual{\texttrademark} group and in 88% in the ILM-Blue{\texttrademark} group. No dye remnants were detected by ophthalmoscopy, and no retinal adverse effects related to the surgery or use of the dyes were observed.CONCLUSION: The 'heavy' dye solutions ILM-Blue{\texttrademark} and MembraneBlue-Dual{\texttrademark} can be injected into a fluid-filled vitreous cavity and may facilitate staining and removal of the ILM and/or ERMs in macular surgery without an additional fluid-air exchange.",

keywords = "Aged, Basement Membrane/pathology, Coloring Agents, Drug Combinations, Epiretinal Membrane/diagnosis, Female, Humans, Indicators and Reagents, Intraocular Pressure/drug effects, Male, Polyethylene Glycols, Prospective Studies, Retinal Diseases/surgery, Rosaniline Dyes, Staining and Labeling/methods, Trypan Blue, Visual Acuity/drug effects, Vitrectomy",

author = "Marc Veckeneer and Andreas Mohr and Essam Alharthi and Rajvardhan Azad and Bashshur, {Ziad F} and Enrico Bertelli and Bejjani, {Riad A} and Brahim Bouassida and Dan Bourla and Crespo, {I{\~n}igo Corc{\'o}stegui} and Charbel Fahed and Faisal Fayyad and Marco Mura and Jerzy Nawrocki and Kelvin Rivett and Scharioth, {Gabor B} and Shkvorchenko, {Dmitry O} and Peter Szurman and {Van Wijck}, Hein and Wong, {Ian Y} and Wong, {David S H} and Johannes Frank and Silke Oellerich and Marieke Bruinsma and Melles, {Gerrit R J}",

note = "{\textcopyright} 2013 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.",

year = "2014",

month = jun,

doi = "10.1111/aos.12208",

language = "English",

volume = "92",

pages = "339--44",

number = "4",

}

Veckeneer, M, Mohr, A, Alharthi, E, Azad, R, Bashshur, ZF, Bertelli, E, Bejjani, RA, Bouassida, B, Bourla, D, Crespo, IC, Fahed, C, Fayyad, F, Mura, M, Nawrocki, J, Rivett, K, Scharioth, GB, Shkvorchenko, DO, Szurman, P, Van Wijck, H, Wong, IY, Wong, DSH, Frank, J, Oellerich, S, Bruinsma, M & Melles, GRJ 2014, 'Novel 'heavy' dyes for retinal membrane staining during macular surgery: multicenter clinical assessment', Acta Ophthalmologica, vol. 92, no. 4, pp. 339-44. https://doi.org/10.1111/aos.12208

TY - JOUR

T1 - Novel 'heavy' dyes for retinal membrane staining during macular surgery

T2 - multicenter clinical assessment

AU - Veckeneer, Marc

AU - Mohr, Andreas

AU - Alharthi, Essam

AU - Azad, Rajvardhan

AU - Bashshur, Ziad F

AU - Bertelli, Enrico

AU - Bejjani, Riad A

AU - Bouassida, Brahim

AU - Bourla, Dan

AU - Crespo, Iñigo Corcóstegui

AU - Fahed, Charbel

AU - Fayyad, Faisal

AU - Mura, Marco

AU - Nawrocki, Jerzy

AU - Rivett, Kelvin

AU - Scharioth, Gabor B

AU - Shkvorchenko, Dmitry O

AU - Szurman, Peter

AU - Van Wijck, Hein

AU - Wong, Ian Y

AU - Wong, David S H

AU - Frank, Johannes

AU - Oellerich, Silke

AU - Bruinsma, Marieke

AU - Melles, Gerrit R J

PY - 2014/6

Y1 - 2014/6

N2 - PURPOSE: To evaluate the feasibility of two novel 'heavy' dye solutions for staining the internal limiting membrane (ILM) and epiretinal membranes (ERMs), without the need for a prior fluid-air exchange, during macular surgery.METHODS: In this prospective nonrandomized multicenter cohort study, the high molecular weight dyes ILM-Blue™ [0.025% brilliant blue G, 4% polyethylene glycol (PEG)] and MembraneBlue-Dual™ (0.15% trypan blue, 0.025% brilliant blue G, 4% PEG) were randomly used in vitrectomy surgeries for macular disease in 127 eyes of 127 patients. Dye enhanced membrane visualization of the ILM and ERMs, 'ease of membrane peeling', visually detectable perioperative retinal damage, postoperative best-corrected visual acuity (BCVA), dye remnants and other unexpected clinical events were documented by 21 surgeons.RESULTS: All surgeries were uneventful, and a clear bluish staining, facilitating the identification, delineation and removal of the ILM and ERMs, was reported in all but five cases. None of the surgeries required a fluid-air exchange to assist the dye application. BCVA at 1 month after surgery improved in 83% of the eyes in the MembraneBlue-Dual™ group and in 88% in the ILM-Blue™ group. No dye remnants were detected by ophthalmoscopy, and no retinal adverse effects related to the surgery or use of the dyes were observed.CONCLUSION: The 'heavy' dye solutions ILM-Blue™ and MembraneBlue-Dual™ can be injected into a fluid-filled vitreous cavity and may facilitate staining and removal of the ILM and/or ERMs in macular surgery without an additional fluid-air exchange.

AB - PURPOSE: To evaluate the feasibility of two novel 'heavy' dye solutions for staining the internal limiting membrane (ILM) and epiretinal membranes (ERMs), without the need for a prior fluid-air exchange, during macular surgery.METHODS: In this prospective nonrandomized multicenter cohort study, the high molecular weight dyes ILM-Blue™ [0.025% brilliant blue G, 4% polyethylene glycol (PEG)] and MembraneBlue-Dual™ (0.15% trypan blue, 0.025% brilliant blue G, 4% PEG) were randomly used in vitrectomy surgeries for macular disease in 127 eyes of 127 patients. Dye enhanced membrane visualization of the ILM and ERMs, 'ease of membrane peeling', visually detectable perioperative retinal damage, postoperative best-corrected visual acuity (BCVA), dye remnants and other unexpected clinical events were documented by 21 surgeons.RESULTS: All surgeries were uneventful, and a clear bluish staining, facilitating the identification, delineation and removal of the ILM and ERMs, was reported in all but five cases. None of the surgeries required a fluid-air exchange to assist the dye application. BCVA at 1 month after surgery improved in 83% of the eyes in the MembraneBlue-Dual™ group and in 88% in the ILM-Blue™ group. No dye remnants were detected by ophthalmoscopy, and no retinal adverse effects related to the surgery or use of the dyes were observed.CONCLUSION: The 'heavy' dye solutions ILM-Blue™ and MembraneBlue-Dual™ can be injected into a fluid-filled vitreous cavity and may facilitate staining and removal of the ILM and/or ERMs in macular surgery without an additional fluid-air exchange.

KW - Aged

KW - Basement Membrane/pathology

KW - Coloring Agents

KW - Drug Combinations

KW - Epiretinal Membrane/diagnosis

KW - Female

KW - Humans

KW - Indicators and Reagents

KW - Intraocular Pressure/drug effects

KW - Male

KW - Polyethylene Glycols

KW - Prospective Studies

KW - Retinal Diseases/surgery

KW - Rosaniline Dyes

KW - Staining and Labeling/methods

KW - Trypan Blue

KW - Visual Acuity/drug effects

KW - Vitrectomy

U2 - 10.1111/aos.12208

DO - 10.1111/aos.12208

M3 - Article

C2 - 23782673

SN - 1755-375X

VL - 92

SP - 339

EP - 344

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

IS - 4

ER -

Novel 'heavy' dyes for retinal membrane staining during macular surgery: multicenter clinical assessment

Abstract

Keywords

Access to Document

Fingerprint

Cite this