Monoclonal antibodies in detection of choroidal melanoma

Peter Ringens; R van Haperen; C Vennegoor; Paulus T V M de Jong; S G van Duinen; D J de Ruiter; A W M van der Kamp

doi:10.1007/Bf02172764

Monoclonal antibodies in detection of choroidal melanoma

Peter Ringens, R van Haperen, C Vennegoor, Paulus T V M de Jong, S G van Duinen, D J de Ruiter, A W M van der Kamp

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Three monoclonal antibodies (MoAbs) prepared against cutaneous melanomas were tested against one group of 12 choroidal melanomas with indirect immunofluorescence in frozen sections. A fourth MoAb was tested in paraffin sections of a second group of 47 choroidal melanomas. One MoAb (NKI-M7) did not react with choroidal melanoma, even though it had a high sensitivity for cutaneous melanoma. A second MoAb (NKI-M6) showed a positive reaction with only 2/12 choroidal melanomas. The third MoAb (NKI/beteb) reacted with all choroidal melanomas, regardless of the cell type. MoAb NKI/C-3 was positive
with 38/47 (81%) choroidal melanomas. We conclude that NKI/C-3 and NKI/beteb have a high sensitivity for both cutaneous and choroidal melanomas in frozen sections. Of these two antibodies NKI/beteb was the most specific for
cutaneous naevi and melanomas.

Original language	English
Pages (from-to)	287-290
Number of pages	4
Journal	Graefe's Archive for Clinical and Experimental Ophthalmology
DOIs	https://doi.org/10.1007/Bf02172764
Publication status	Published - 1989

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title = "Monoclonal antibodies in detection of choroidal melanoma",

abstract = "Three monoclonal antibodies (MoAbs) prepared against cutaneous melanomas were tested against one group of 12 choroidal melanomas with indirect immunofluorescence in frozen sections. A fourth MoAb was tested in paraffin sections of a second group of 47 choroidal melanomas. One MoAb (NKI-M7) did not react with choroidal melanoma, even though it had a high sensitivity for cutaneous melanoma. A second MoAb (NKI-M6) showed a positive reaction with only 2/12 choroidal melanomas. The third MoAb (NKI/beteb) reacted with all choroidal melanomas, regardless of the cell type. MoAb NKI/C-3 was positive with 38/47 (81%) choroidal melanomas. We conclude that NKI/C-3 and NKI/beteb have a high sensitivity for both cutaneous and choroidal melanomas in frozen sections. Of these two antibodies NKI/beteb was the most specific for cutaneous naevi and melanomas.",

author = "Peter Ringens and {van Haperen}, R and C Vennegoor and {de Jong}, {Paulus T V M} and {van Duinen}, {S G} and {de Ruiter}, {D J} and {van der Kamp}, {A W M}",

year = "1989",

doi = "10.1007/Bf02172764",

language = "English",

pages = "287--290",

}

TY - JOUR

T1 - Monoclonal antibodies in detection of choroidal melanoma

AU - Ringens, Peter

AU - van Haperen, R

AU - Vennegoor, C

AU - de Jong, Paulus T V M

AU - van Duinen, S G

AU - de Ruiter, D J

AU - van der Kamp, A W M

PY - 1989

Y1 - 1989

N2 - Three monoclonal antibodies (MoAbs) prepared against cutaneous melanomas were tested against one group of 12 choroidal melanomas with indirect immunofluorescence in frozen sections. A fourth MoAb was tested in paraffin sections of a second group of 47 choroidal melanomas. One MoAb (NKI-M7) did not react with choroidal melanoma, even though it had a high sensitivity for cutaneous melanoma. A second MoAb (NKI-M6) showed a positive reaction with only 2/12 choroidal melanomas. The third MoAb (NKI/beteb) reacted with all choroidal melanomas, regardless of the cell type. MoAb NKI/C-3 was positive with 38/47 (81%) choroidal melanomas. We conclude that NKI/C-3 and NKI/beteb have a high sensitivity for both cutaneous and choroidal melanomas in frozen sections. Of these two antibodies NKI/beteb was the most specific for cutaneous naevi and melanomas.

AB - Three monoclonal antibodies (MoAbs) prepared against cutaneous melanomas were tested against one group of 12 choroidal melanomas with indirect immunofluorescence in frozen sections. A fourth MoAb was tested in paraffin sections of a second group of 47 choroidal melanomas. One MoAb (NKI-M7) did not react with choroidal melanoma, even though it had a high sensitivity for cutaneous melanoma. A second MoAb (NKI-M6) showed a positive reaction with only 2/12 choroidal melanomas. The third MoAb (NKI/beteb) reacted with all choroidal melanomas, regardless of the cell type. MoAb NKI/C-3 was positive with 38/47 (81%) choroidal melanomas. We conclude that NKI/C-3 and NKI/beteb have a high sensitivity for both cutaneous and choroidal melanomas in frozen sections. Of these two antibodies NKI/beteb was the most specific for cutaneous naevi and melanomas.

U2 - 10.1007/Bf02172764

DO - 10.1007/Bf02172764

M3 - Article

SN - 0721-832X

SP - 287

EP - 290

JO - Graefe's Archive for Clinical and Experimental Ophthalmology

JF - Graefe's Archive for Clinical and Experimental Ophthalmology

ER -

Monoclonal antibodies in detection of choroidal melanoma

Abstract

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