Latent acyclovir-resistant herpes simplex virus type 1 in trigeminal ganglia of immunocompetent individuals

Monique van Velzen; Freek B van Loenen; Roland J W Meesters; Miranda de Graaf; Lies Remeijer; Theo M Luider; Albert D M E Osterhaus; Georges M G M Verjans

doi:10.1093/infdis/jis237

Latent acyclovir-resistant herpes simplex virus type 1 in trigeminal ganglia of immunocompetent individuals

Monique van Velzen, Freek B van Loenen, Roland J W Meesters, Miranda de Graaf, Lies Remeijer, Theo M Luider, Albert D M E Osterhaus, Georges M G M Verjans

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Research output: Contribution to journal › Article › Research › peer-review

Abstract

Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.

Original language	English
Pages (from-to)	1539-43
Number of pages	5
Journal	The Journal of infectious diseases
Volume	205
Issue number	10
DOIs	https://doi.org/10.1093/infdis/jis237
Publication status	Published - 15 May 2012

Keywords

Acyclovir/pharmacology
Aged
Aged, 80 and over
Animals
Antiviral Agents/pharmacology
Autopsy
Base Sequence
COS Cells
Chlorocebus aethiops
DNA, Viral/genetics
Drug Resistance, Viral/genetics
Female
Herpes Simplex/drug therapy
Herpesvirus 1, Human/drug effects
Humans
Immunocompetence
Male
Molecular Sequence Data
Mutation
Phylogeny
Prevalence
Sequence Analysis, DNA
Thymidine Kinase/chemistry
Trigeminal Ganglion/virology
Virus Latency

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10.1093/infdis/jis237

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title = "Latent acyclovir-resistant herpes simplex virus type 1 in trigeminal ganglia of immunocompetent individuals",

abstract = "Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.",

keywords = "Acyclovir/pharmacology, Aged, Aged, 80 and over, Animals, Antiviral Agents/pharmacology, Autopsy, Base Sequence, COS Cells, Chlorocebus aethiops, DNA, Viral/genetics, Drug Resistance, Viral/genetics, Female, Herpes Simplex/drug therapy, Herpesvirus 1, Human/drug effects, Humans, Immunocompetence, Male, Molecular Sequence Data, Mutation, Phylogeny, Prevalence, Sequence Analysis, DNA, Thymidine Kinase/chemistry, Trigeminal Ganglion/virology, Virus Latency",

author = "{van Velzen}, Monique and {van Loenen}, {Freek B} and Meesters, {Roland J W} and {de Graaf}, Miranda and Lies Remeijer and Luider, {Theo M} and Osterhaus, {Albert D M E} and Verjans, {Georges M G M}",

year = "2012",

month = may,

day = "15",

doi = "10.1093/infdis/jis237",

language = "English",

volume = "205",

pages = "1539--43",

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T1 - Latent acyclovir-resistant herpes simplex virus type 1 in trigeminal ganglia of immunocompetent individuals

AU - van Velzen, Monique

AU - van Loenen, Freek B

AU - Meesters, Roland J W

AU - de Graaf, Miranda

AU - Remeijer, Lies

AU - Luider, Theo M

AU - Osterhaus, Albert D M E

AU - Verjans, Georges M G M

PY - 2012/5/15

Y1 - 2012/5/15

N2 - Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.

AB - Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.

KW - Acyclovir/pharmacology

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Antiviral Agents/pharmacology

KW - Autopsy

KW - Base Sequence

KW - COS Cells

KW - Chlorocebus aethiops

KW - DNA, Viral/genetics

KW - Drug Resistance, Viral/genetics

KW - Female

KW - Herpes Simplex/drug therapy

KW - Herpesvirus 1, Human/drug effects

KW - Humans

KW - Immunocompetence

KW - Male

KW - Molecular Sequence Data

KW - Mutation

KW - Phylogeny

KW - Prevalence

KW - Sequence Analysis, DNA

KW - Thymidine Kinase/chemistry

KW - Trigeminal Ganglion/virology

KW - Virus Latency

U2 - 10.1093/infdis/jis237

DO - 10.1093/infdis/jis237

M3 - Article

C2 - 22457282

SN - 0022-1899

VL - 205

SP - 1539

EP - 1543

JO - The Journal of infectious diseases

JF - The Journal of infectious diseases

IS - 10

ER -

Latent acyclovir-resistant herpes simplex virus type 1 in trigeminal ganglia of immunocompetent individuals

Abstract

Keywords

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