Intraocular T cells of patients with herpes simplex virus (HSV)-induced acute retinal necrosis recognize HSV tegument proteins VP11/12 and VP13/14

Georges M G M Verjans, M E Dings, John McLauchlan, Alex van der Kooi, Peter Hoogerhout, Humphrey F Brugghe, Hans A M Timmermans, G S Baarsma, A D Osterhaus

Research output: Contribution to journalArticleResearchpeer-review

Abstract

It has previously been shown that T cells specific for the triggering virus infiltrate the eye of patients with herpes simplex virus type 1 (HSV-1)-induced acute retinal necrosis (ARN). The T cells were mainly directed against 0.67-0.73 HSV-1 map region encoded antigens. The fine specificities of genetically different T cell clones (TCC), obtained from affected eyes of 3 patients with HSV-induced ARN and reactive toward this genomic region of HSV-1, were analyzed with recombinant HSV viruses and synthetic peptides. For 1 patient, the HSV-1 UL46 gene encoded tegument protein VP11/12 was identified as the target antigen. Two separate CD4(+) T cell epitopes were defined in VP11/12. TCC from the other 2 patients recognized the HSV-1 UL47 gene encoded tegument protein VP13/14. Two separate CD4(+) VP13/14 T cell epitopes were identified in these patients. Analysis of the data indicates that HSV-1 VP11/12 and VP13/14 are major target antigens for T cells obtained from vitreous fluid samples of the HSV-induced ARN patients studied.

Original languageEnglish
Pages (from-to)923-7
Number of pages5
JournalThe Journal of infectious diseases
Volume182
Issue number3
DOIs
Publication statusPublished - Sep 2000

Keywords

  • Antigens, Viral
  • Cells, Cultured
  • Cytotoxicity, Immunologic
  • Epitopes
  • Eye/immunology
  • Herpesvirus 1, Human/genetics
  • Humans
  • Lymphocyte Activation
  • Phenotype
  • Receptors, Antigen, T-Cell, alpha-beta/immunology
  • Retinal Necrosis Syndrome, Acute/immunology
  • T-Lymphocytes/immunology
  • Viral Fusion Proteins/immunology
  • Viral Proteins

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