Immune electron microscopy and a cultural test in the diagnosis of adenovirus ocular infection

G Van Rij; L Klepper; E Peperkamp; G J Schaap

doi:10.1136/bjo.66.5.317

Immune electron microscopy and a cultural test in the diagnosis of adenovirus ocular infection

G Van Rij, L Klepper, E Peperkamp, G J Schaap

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Immune electron microscopy (IEM) and virus isolation in cell culture were compared in the diagnosis of adenovirus ocular infection during an outbreak of the disease in 1979. Eleven of 14 patients with a keratoconjunctivitis clinically indicative of adenovirus infection had IEM evidence of adenovirus infection or had the virus isolated from ocular swabs. The IEM was positive in 8 patients. Virus as isolated from 10 patients. IEM was positive in one culture negative patient. Since IEM provides a rapid and sensitive method for the detection of adenovirus in human tears, it may be a valuable diagnostic tool for the clinician.

Original language	English
Pages (from-to)	317-9
Number of pages	3
Journal	British Journal of Ophthalmology
Volume	66
Issue number	5
DOIs	https://doi.org/10.1136/bjo.66.5.317
Publication status	Published - May 1982

Keywords

Adenoviridae Infections/diagnosis
Adenovirus Infections, Human/diagnosis
Adenoviruses, Human/isolation & purification
Humans
Keratoconjunctivitis/diagnosis
Microscopy, Electron
Tears/microbiology

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10.1136/bjo.66.5.317

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title = "Immune electron microscopy and a cultural test in the diagnosis of adenovirus ocular infection",

abstract = "Immune electron microscopy (IEM) and virus isolation in cell culture were compared in the diagnosis of adenovirus ocular infection during an outbreak of the disease in 1979. Eleven of 14 patients with a keratoconjunctivitis clinically indicative of adenovirus infection had IEM evidence of adenovirus infection or had the virus isolated from ocular swabs. The IEM was positive in 8 patients. Virus as isolated from 10 patients. IEM was positive in one culture negative patient. Since IEM provides a rapid and sensitive method for the detection of adenovirus in human tears, it may be a valuable diagnostic tool for the clinician.",

keywords = "Adenoviridae Infections/diagnosis, Adenovirus Infections, Human/diagnosis, Adenoviruses, Human/isolation & purification, Humans, Keratoconjunctivitis/diagnosis, Microscopy, Electron, Tears/microbiology",

author = "{Van Rij}, G and L Klepper and E Peperkamp and Schaap, {G J}",

year = "1982",

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doi = "10.1136/bjo.66.5.317",

language = "English",

volume = "66",

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T1 - Immune electron microscopy and a cultural test in the diagnosis of adenovirus ocular infection

AU - Van Rij, G

AU - Klepper, L

AU - Peperkamp, E

AU - Schaap, G J

PY - 1982/5

Y1 - 1982/5

N2 - Immune electron microscopy (IEM) and virus isolation in cell culture were compared in the diagnosis of adenovirus ocular infection during an outbreak of the disease in 1979. Eleven of 14 patients with a keratoconjunctivitis clinically indicative of adenovirus infection had IEM evidence of adenovirus infection or had the virus isolated from ocular swabs. The IEM was positive in 8 patients. Virus as isolated from 10 patients. IEM was positive in one culture negative patient. Since IEM provides a rapid and sensitive method for the detection of adenovirus in human tears, it may be a valuable diagnostic tool for the clinician.

AB - Immune electron microscopy (IEM) and virus isolation in cell culture were compared in the diagnosis of adenovirus ocular infection during an outbreak of the disease in 1979. Eleven of 14 patients with a keratoconjunctivitis clinically indicative of adenovirus infection had IEM evidence of adenovirus infection or had the virus isolated from ocular swabs. The IEM was positive in 8 patients. Virus as isolated from 10 patients. IEM was positive in one culture negative patient. Since IEM provides a rapid and sensitive method for the detection of adenovirus in human tears, it may be a valuable diagnostic tool for the clinician.

KW - Adenoviridae Infections/diagnosis

KW - Adenovirus Infections, Human/diagnosis

KW - Adenoviruses, Human/isolation & purification

KW - Humans

KW - Keratoconjunctivitis/diagnosis

KW - Microscopy, Electron

KW - Tears/microbiology

U2 - 10.1136/bjo.66.5.317

DO - 10.1136/bjo.66.5.317

M3 - Article

C2 - 6280747

SN - 0007-1161

VL - 66

SP - 317

EP - 319

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

IS - 5

ER -

Immune electron microscopy and a cultural test in the diagnosis of adenovirus ocular infection

Abstract

Keywords

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