Endothelial cell density after posterior lamellar keratoplasty (Melles techniques): 3 years follow-up

Bart Van Dooren, Paul G H Mulder, Carla P Nieuwendaal, W H Beekhuis, Gerrit R J Melles

    Research output: Contribution to journalArticleResearchpeer-review


    PURPOSE: To report the midterm endothelial cell density measurements after posterior lamellar keratoplasty (Melles techniques).

    DESIGN: Cohort study.

    METHODS: Fifteen consecutive eyes of 15 patients in whom a posterior lamellar keratoplasty procedure was performed for pseudophakic bullous keratopathy or Fuchs' endothelial dystrophy were evaluated. In 11 corneas the donor tissue was inserted through a 9.0-mm sclerocorneal pocket incision (technique A); in four cases the donor was folded and inserted through a 5.0-mm incision (technique B). Specular microscopy was performed at 6, 12, 24, and 36 months after surgery, to measure the endothelial cell density.

    RESULTS: Mean postoperative endothelial cell density averaged 2,126 cells/mm(2) (+/-548) at 6 months, 1,859 cells/mm(2) (+/-477) at 12 months, 1,385 cells/mm(2) (+/-451) at 24 months, and 1,047 cells/mm(2) (+/-425) at 36 months.

    CONCLUSION: In posterior lamellar keratoplasty, the donor corneal endothelium showed a decrease in cell density similar to that after conventional full-thickness penetrating keratoplasty.

    Original languageEnglish
    Pages (from-to)211-7
    Number of pages7
    JournalAmerican Journal of Ophthalmology
    Issue number2
    Publication statusPublished - Aug 2004


    • Aged
    • Cell Count
    • Cohort Studies
    • Corneal Diseases/surgery
    • Corneal Transplantation
    • Endothelium, Corneal/pathology
    • Female
    • Follow-Up Studies
    • Humans
    • Male
    • Postoperative Complications


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