Double vital staining using trypan blue and infracyanine green in macular pucker surgery

P Stalmans, E J Feron, R Parys-Van Ginderdeuren, A Van Lommel, G R J Melles, M Veckeneer

Research output: Contribution to journalArticleResearchpeer-review


AIMS: To study the clinical properties of double vital staining in premacular fibrosis, facilitating complete removal of all epiretinal tissue.

METHODS: In a two step surgery, the epiretinal pucker was removed after staining with trypan blue, whereafter the inner limiting membrane was peeled after staining with infracyanine green.

RESULTS: In all 30 patients, a separate epiretinal layer and inner limiting membrane were removed from the macular area. Pathological examination showed different histological properties of the removed layers. An increased visual acuity was measured in 26 patients, and a slightly decreased visual acuity in one patient.

CONCLUSION: The described double staining technique could be a novel valuable tool that may help to achieve optimal anatomical and functional recovery after surgery for premacular fibrosis

Original languageEnglish
Pages (from-to)713-716
Number of pages4
JournalBritish Journal of Ophthalmology
Issue number6
Publication statusPublished - Jun 2003


  • Aged
  • Aged, 80 and over
  • Coloring Agents
  • Female
  • Humans
  • Immunohistochemistry
  • Indocyanine Green/analogs & derivatives
  • Macula Lutea/surgery
  • Male
  • Middle Aged
  • Retinal Diseases/diagnosis
  • Staining and Labeling/methods
  • Trypan Blue
  • Visual Acuity


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