Corneal epithelial Cl-dependent pump quantified

W H Beekhuis, B E McCarey

    Research output: Contribution to journalArticleResearchpeer-review


    Pairs of rabbit corneas were perfused and corneal thickness monitored. The epithelial side of the preparation, and after stabilization also the endothelial side, was covered with silicone oil. A constant thinning of the cornea was observed at a rate of 11.1 microns hr-1 (= 1.0 micron L h-1 cm-2) S.D. 2.1, n = 9. This thinning was also present after endothelial removal: 12.3 microns hr-1, S.D. 1.6 (n = 5). Epithelial abrasion virtually abolished the thinning (2.5 microns hr-1, S.D. 1.89, n = 4), as did cooling from 34 to 0 degrees C. When the preparation was perfused with a Cl-free solution (SO4(2-) instead of Cl-, corrected for osmolarity with sucrose) no significant thinning of the preparation was observed (2.4 microns hr-1, S.D. 2.49, n = 4) after covering both surfaces with silicone oil. This simple set of experiments quantified the epithelial pump mechanism. The epithelial pump rate of about 1.2 microns L hr-1 cm-2 has to be taken into account when endothelial pump rates are measured in the in vitro preparation with intact epithelium.

    Original languageEnglish
    Pages (from-to)707-11
    Number of pages5
    JournalExperimental Eye Research
    Issue number5
    Publication statusPublished - Nov 1986


    • Animals
    • Biological Transport, Active
    • Chlorides/metabolism
    • Cold Temperature
    • Cornea/metabolism
    • Epithelium/metabolism
    • In Vitro Techniques
    • Rabbits
    • Silicone Oils


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