Chromosome 3 intratumor heterogeneity in uveal melanoma

Hanneke W Mensink, Jolanda Vaarwater, Emine Kiliç, Nicole C Naus, Neeltje Mooy, Gre Luyten, Hennie T Brüggenwirth, Dion Paridaens, Annelies de Klein

Research output: Contribution to journalArticleResearchpeer-review


PURPOSE: To investigate the presence of focal or diffuse heterogeneity of monosomy 3 in uveal melanoma, by using fluorescence in situ hybridization (FISH).

METHODS: Direct interphase FISH in a series of 151 uveal melanomas revealed 82 tumors with loss of chromosome 3. Tumors with monosomy 3 were suspected to be heterogeneous if there were low percentages of monosomy 3, triploid clones, inconsistencies between FISH on centromere 3 and the long arm of chromosome 3, or discrepancies between fine-needle-aspiration biopsies (FNABs) and the main tumor. These tumors (n=16), all choroidal melanomas, were selected and analyzed for intratumor heterogeneity by using FISH on paraffin-embedded tissue sections.

RESULTS: Different sections of each tumor were evaluated with FISH: 6 tumors showed monosomy 3 in the same percentage throughout the tumor, and 10 showed multiple clones with different percentages of monosomy 3. However, these tumors did not show focal heterogeneity with respect to chromosome 3 status, and differences in monosomy 3 distribution between the base and apex of the tumor could not be identified.

CONCLUSIONS: Although a small number of uveal melanomas show heterogeneity for chromosome 3, it does not affect survival. In the presence of triploid clones, the loss of chromosome 3 is more difficult to interpret. In general, tumor biopsies in uveal melanoma provide an accurate prediction of the patient's prognosis.

Original languageEnglish
Pages (from-to)500-4
Number of pages5
JournalInvestigative ophthalmology & visual science
Issue number2
Publication statusPublished - Feb 2009


  • Choroid Neoplasms/genetics
  • Chromosomes, Human, Pair 3/genetics
  • Female
  • Genetic Heterogeneity
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Melanoma/genetics
  • Middle Aged
  • Monosomy/genetics
  • Prognosis


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