ARHGEF12 influences the risk of glaucoma by increasing intraocular pressure

Henriët Springelkamp, Adriana I Iglesias, Gabriel Cuellar-Partida, Najaf Amin, Kathryn P Burdon, Elisabeth M van Leeuwen, Puya Gharahkhani, Aniket Mishra, Sven J van der Lee, Alex W Hewitt, Fernando Rivadeneira, Ananth C Viswanathan, Roger C W Wolfs, Nicholas G Martin, Wishal D Ramdas, Leonieke M van Koolwijk, Craig E Pennell, Johannes R Vingerling, Jenny E Mountain, André G UitterlindenAlbert Hofman, Paul Mitchell, Hans G Lemij, Jie Jin Wang, Caroline C W Klaver, David A Mackey, Jamie E Craig, Cornelia M van Duijn, Stuart MacGregor

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Primary open-angle glaucoma (POAG) is a blinding disease. Two important risk factors for this disease are a positive family history and elevated intraocular pressure (IOP), which is also highly heritable. Genes found to date associated with IOP and POAG are ABCA1, CAV1/CAV2, GAS7 and TMCO1. However, these genes explain only a small part of the heritability of IOP and POAG. We performed a genome-wide association study of IOP in the population-based Rotterdam Study I and Rotterdam Study II using single nucleotide polymorphisms (SNPs) imputed to 1000 Genomes. In this discovery cohort (n = 8105), we identified a new locus associated with IOP. The most significantly associated SNP was rs58073046 (β = 0.44, P-value = 1.87 × 10(-8), minor allele frequency = 0.12), within the gene ARHGEF12. Independent replication in five population-based studies (n = 7471) resulted in an effect size in the same direction that was significantly associated (β = 0.16, P-value = 0.04). The SNP was also significantly associated with POAG in two independent case-control studies [n = 1225 cases and n = 4117 controls; odds ratio (OR) = 1.53, P-value = 1.99 × 10(-8)], especially with high-tension glaucoma (OR = 1.66, P-value = 2.81 × 10(-9); for normal-tension glaucoma OR = 1.29, P-value = 4.23 × 10(-2)). ARHGEF12 plays an important role in the RhoA/RhoA kinase pathway, which has been implicated in IOP regulation. Furthermore, it binds to ABCA1 and links the ABCA1, CAV1/CAV2 and GAS7 pathway to Mendelian POAG genes (MYOC, OPTN, WDR36). In conclusion, this study identified a novel association between IOP and ARHGEF12.

Original languageEnglish
Pages (from-to)2689-99
Number of pages11
JournalHuman Molecular Genetics
Volume24
Issue number9
Publication statusPublished - 1 May 2015

Keywords

  • Aged
  • Female
  • Gene Expression
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Genome-Wide Association Study
  • Glaucoma/epidemiology
  • Glaucoma, Open-Angle/genetics
  • Humans
  • Intraocular Pressure/genetics
  • Male
  • Meta-Analysis as Topic
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Protein Interaction Mapping
  • Protein Interaction Maps
  • Rho Guanine Nucleotide Exchange Factors/genetics

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