Abstract
In conventional phase-resolved OCT blood flow is detected from phase changes between successive A-scans. Especially in high-speed OCT systems this results in a short evaluation time interval. This method is therefore often unable to visualize complete vascular networks since low flow velocities cause insufficient phase changes. This problem was solved by comparing B-scans instead of successive A-scans to enlarge the time interval. In this paper a detailed phase-noise analysis of our OCT system is presented in order to calculate the optimal time intervals for visualization of the vasculature of the human retina and choroid. High-resolution images of the vasculature of a healthy volunteer taken with various time intervals are presented to confirm this analysis. The imaging was performed with a backstitched B-scan in which pairs of small repeated B-scans are stitched together to independently control the time interval and the imaged lateral field size. A time interval of ≥ 2.5 ms was found effective to image the retinal vasculature down to the capillary level. The higher flow velocities of the choroid allowed a time interval of 0.64 ms to reveal its dense vasculature. Finally we analyzed depth-resolved histograms of volumetric phase-difference data to assess changes in amount of blood flow with depth. This analysis indicated different flow regimes in the retina and the choroid.
Original language | English |
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Pages (from-to) | 20516-34 |
Number of pages | 19 |
Journal | Optics Express |
Volume | 20 |
Issue number | 18 |
DOIs | |
Publication status | Published - 27 Aug 2012 |
Keywords
- Algorithms
- Angiography/instrumentation
- Ciliary Arteries/anatomy & histology
- Humans
- Image Enhancement/instrumentation
- Reproducibility of Results
- Retinal Vessels/anatomy & histology
- Retinoscopes
- Sensitivity and Specificity
- Tomography, Optical Coherence/instrumentation