An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis

Luana Ugahary, Wendy van de Sande, Jan C van Meurs, Alex van Belkum

Research output: Contribution to journalLetterResearchpeer-review

Abstract

General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

Original languageEnglish
Pages (from-to)5403-5
Number of pages3
JournalJournal of Clinical Microbiology
Volume42
Issue number11
DOIs
Publication statusPublished - Nov 2004

Keywords

  • Anti-Infective Agents, Local/chemistry
  • DNA, Bacterial/analysis
  • Endophthalmitis/diagnosis
  • Equipment Contamination
  • Eye Infections, Bacterial/diagnosis
  • Humans
  • Povidone-Iodine/chemistry
  • Pseudomonas/chemistry
  • Vitrectomy

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