An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis

Luana Ugahary; Wendy van de Sande; Jan C van Meurs; Alex van Belkum

doi:10.1128/JCM.42.11.5403-5405.2004

An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis

Luana Ugahary, Wendy van de Sande, Jan C van Meurs, Alex van Belkum

Vitreoretinal surgery

Research output: Contribution to journal › Letter › peer-review

Abstract

General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

Original language	English
Pages (from-to)	5403-5
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	42
Issue number	11
DOIs	https://doi.org/10.1128/JCM.42.11.5403-5405.2004
Publication status	Published - Nov 2004

Keywords

Anti-Infective Agents, Local/chemistry
DNA, Bacterial/analysis
Endophthalmitis/diagnosis
Equipment Contamination
Eye Infections, Bacterial/diagnosis
Humans
Povidone-Iodine/chemistry
Pseudomonas/chemistry
Vitrectomy

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10.1128/JCM.42.11.5403-5405.2004

Cite this

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title = "An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis",

abstract = "General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.",

keywords = "Anti-Infective Agents, Local/chemistry, DNA, Bacterial/analysis, Endophthalmitis/diagnosis, Equipment Contamination, Eye Infections, Bacterial/diagnosis, Humans, Povidone-Iodine/chemistry, Pseudomonas/chemistry, Vitrectomy",

author = "Luana Ugahary and {van de Sande}, Wendy and {van Meurs}, {Jan C} and {van Belkum}, Alex",

year = "2004",

month = nov,

doi = "10.1128/JCM.42.11.5403-5405.2004",

language = "English",

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TY - JOUR

T1 - An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis

AU - Ugahary, Luana

AU - van de Sande, Wendy

AU - van Meurs, Jan C

AU - van Belkum, Alex

PY - 2004/11

Y1 - 2004/11

N2 - General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

AB - General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

KW - Anti-Infective Agents, Local/chemistry

KW - DNA, Bacterial/analysis

KW - Endophthalmitis/diagnosis

KW - Equipment Contamination

KW - Eye Infections, Bacterial/diagnosis

KW - Humans

KW - Povidone-Iodine/chemistry

KW - Pseudomonas/chemistry

KW - Vitrectomy

U2 - 10.1128/JCM.42.11.5403-5405.2004

DO - 10.1128/JCM.42.11.5403-5405.2004

M3 - Letter

C2 - 15528756

SN - 0095-1137

VL - 42

SP - 5403

EP - 5405

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis

Abstract

Keywords

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