A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes

C Rousou; P Hoogenboom; K A van Overdam; G Storm; J Dorrestijn; E Mastrobattista

doi:10.1016/j.exer.2019.02.003

A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes

C Rousou, P Hoogenboom, K A van Overdam, G Storm, J Dorrestijn, E Mastrobattista

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.

Original language	English
Pages (from-to)	171-177
Number of pages	7
Journal	Eye (London, England)
Volume	181
DOIs	https://doi.org/10.1016/j.exer.2019.02.003
Publication status	Published - Apr 2019

Keywords

Animals
Eye/blood supply
In Vitro Techniques/methods
Models, Animal
Ophthalmic Artery
Perfusion/methods
Retinal Vessels
Swine

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10.1016/j.exer.2019.02.003Licence: CC BY

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title = "A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes",

abstract = "Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.",

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author = "C Rousou and P Hoogenboom and {van Overdam}, {K A} and G Storm and J Dorrestijn and E Mastrobattista",

year = "2019",

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doi = "10.1016/j.exer.2019.02.003",

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AU - Rousou, C

AU - Hoogenboom, P

AU - van Overdam, K A

AU - Storm, G

AU - Dorrestijn, J

AU - Mastrobattista, E

PY - 2019/4

Y1 - 2019/4

N2 - Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.

AB - Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.

KW - Animals

KW - Eye/blood supply

KW - In Vitro Techniques/methods

KW - Models, Animal

KW - Ophthalmic Artery

KW - Perfusion/methods

KW - Retinal Vessels

KW - Swine

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DO - 10.1016/j.exer.2019.02.003

M3 - Article

C2 - 30735657

SN - 0950-222X

VL - 181

SP - 171

EP - 177

JO - Eye (London, England)

JF - Eye (London, England)

ER -

A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes

Abstract

Keywords

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